Bin2 phosphorylation mutant
WebApr 20, 2024 · In this study, we show that the GSK3 kinase BIN2 physically interacts with CO and the gain-of-function mutant bin2-1 displays late flowering phenotype through down-regulation of FT transcription. Genetic analyses show that BIN2 genetically acts upstream of CO in regulating flowering time. WebMay 14, 2024 · We thus tested whether BIN2 mediates the phosphorylation of BZR1 on high sugar medium. It is reported that BIN2 interacts directly with BZR1 through a 12-amino …
Bin2 phosphorylation mutant
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WebApr 6, 2015 · AtSK21/BIN2 phosphorylation of ARF2 alleviates DNA binding and repressor activity of ARF2, ... Notably, mutant bin2-1 protein accumulates in greater quantities than wild-type AtSK21/BIN2 in plant cells (Peng et al., 2008). The BR biosynthetic inhibitor BRZ induces the accumulation of AtSK21/BIN2 in plant cells. WebJul 11, 2002 · BIN2 encodes a member of the glycogen synthase kinase-3 (GSK3)-like kinases ( 14, 15 ). The increased kinase activity by the semidominant bin2–1 mutation and the phenotypes of transgenic plants …
WebMar 30, 2024 · The BIN2 gain-of-function mutant bin2-1 displays an enhanced photomorphogenic phenotype in both far-red and red lights. phyA-enhanced … WebBIN2 integrates multiple signaling pathways by phosphorylating a range of proteins including the components in light signaling. For example, PIF3, PIF4, and PIF5 can be phosphorylated by BIN2 and then degraded via the 26S proteasome pathway to control transcription and hypocotyl growth ( Bernardo-García et al., 2014; Ling et al., 2024 ).
To further identify the mechanism by which HY5 regulated BIN2 in the light to destabilize BZR1, we first checked whether HY5 regulated the mRNA and/or protein levels of BIN2. As shown in Supplementary Fig. 5, both the mRNA and protein levels of BIN2 were similar in hy5 and WT, suggesting that HY5 does not … See more To investigate the cross-talk between light and BR signaling, we performed yeast two-hybrid analyses and found that BIN2, the key negative … See more Next, we investigated whether HY5 could genetically interact with BIN2 by crossing hy5 into bin2-1, a gain-of-function mutant of BIN228. We found that the hypocotyls of double mutant … See more To understand which amino acid residues of HY5 were important for its interaction with BIN2, we screened BIN2-interacting sites in the HY5 LZ region. As shown in Fig. 5a, changing HY5 Leu137 (L137) to Asp (named HY5-M) … See more Previous reports have suggested that HY5 and BIN2 have similar effects on BZR1 protein accumulation20,27. To gain further insight into their … See more WebMar 21, 2024 · GeneCards Summary for BIN2 Gene. BIN2 (Bridging Integrator 2) is a Protein Coding gene. Among its related pathways are Innate Immune System and …
WebNov 9, 2024 · GSK3-like Kinases Negatively Regulate Salt Stress Tolerance. To determine if GSK3-like kinases function in the plant salt stress response, we assessed the salt sensitivity of a BIN2, BIL1, and BIL2 triple loss-of-function mutant (bin2-3bil1bil2, abbreviated 3b) (Yan et al., 2009).Seedlings of the 3b mutant had a significantly shorter primary root …
WebNov 11, 2024 · e, Western blot with the custom-made p-BIN2 antibody on BIN2-WT or BIN2-AA (T423A and S424A non-phosphorylable mutant)-expressing A2780 cells showed that the p-BIN2 antibody detected an expected ... diamond cars hamstead villageWebmany other phosphorylation sites remain unknown. In addition, overexpression of Y200 phosphatase BSU1 only partially rescued the BRI1 mutant bri1-116 (6), indicating that other mechanisms may also be involved in the regulation of BIN2 activity. Fur-thermore, BRs can regulate BIN2 degradation mediated by the 26S proteasome by unknown mechanisms ... circor warren jobsWebDec 20, 2012 · Mutant MBP-mBZR1 protein containing bzr1–1Dmuta- tion alsophosphorylated GST-BIN2.Consistent fastermobility mBZR1-CFPproteins im-munoblot analysis transgenicplants, bzr1–1D mutation ap- peared vitro-phosphorylatedMBP-mBZR1 protein hadlittle effect unphosphorylatedBZR1 fusion protein (Fig. 2B), suggesting … circor thaneWebBIN2 (brassinosteroid-insensitive 2) kinase, a GSK3-like kinase functioning as a negative regulator in BR signaling, directly phosphorylated RAPTOR1B (regulatory-associated … diamond carrot wizard101WebMay 25, 2024 · The disease symptoms caused by V. dahliae were more severe in the gain-of-function mutant bin2-1 seedlings compared with the control plants Col-0. The loss-of … diamond cartier watch aliexpressWebApr 11, 2024 · Highlights. As a major environmental factor impacting the seasonal growth and geographical distribution of plants, temperature change significantly affects crop quality and productivity. Ethylene is a gaseous hormone with an important role in plant growth, development, and multiple stress responses, including heat and cold. Temperature stress ... diamond car shampooWebIt was shown that the BIN2-catalyzed phosphorylation of BSK3 promotes the interactions of BSK3 with the BR receptor BRI1 (Brassinosteroid-Insensitive1) ... elimination of GSK3-like kinases resulted in an increased ratio of SE/CC whereas overexpression of a gain-of-function mutant variant bin2-1 slightly decreased the SE/CC ratio, ... circor yahoo finance