Ph-ncas9-ppe
WebbpH-nCas9-PPE-V2 pSpG-PPE pH-ePPE ePPE pRN120 Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn … Webb21 feb. 2024 · Cas9 protein (1 μg) and sgRNA (1 μg) were mixed with the purified target DNA (100–150 ng) in Cas9 reaction buffer (20 mM HEPES, pH 7.5, 150 mM KCl, 10 mM MgCl 2, 0.5 mM DTT) in a total volume of 20 μl, followed by digestion at 37 °C for 0.5–1 h.
Ph-ncas9-ppe
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Webb18 dec. 2024 · A fragment containing these two sgRNA expression cassettes was PCR amplified and cloned into the HindIII-digested pH-nCas9-PBE binary plasmid (Fig. S1b). All recombinant plasmids were subjected to DNA sequencing for verification. Primers used for molecular cloning are listed in Table S1. Generation of transgenic plants WebbpH-nCas9-PBE DescriptionAlias:L-98163 Promoter:OsU3,UBI Replicator:pVS1 Clone strain:Stbl3 Culture conditions:37centigrade Host
WebbFor sgRNA expression in monocotyledons protoplasts. Loading... 140447. pH-nCas9-PPE. For plant prime editing in rice plants or monocotyledons protoplasts. Loading... 140445. … WebbpOsU3 pTaU3 pH-nCas9-PPE-V2/pH-ePPE Forward primer (5'-3'): + Spacer sequence + Reverse primer (5'-3'): + PBS and RT template sequence + Submit >> clear. Help document. There are 35458 Visits, latest from 52.167.144.144 ...
WebbThe pOsU3, pTaU3, and pTaU6 vectors are recommended for particle bombardment and protoplast transformation experiments. The pH-nCas9-PPE-V2 vector is recommended … Webb15 apr. 2024 · To construct the pH-nCas9 (H840A) vector for the control group, the M-MLV sequence of pH-nCas9-PPE was removed using a ClonExpress II Cloning Kit. The PE3 …
WebbLin Q, Zong Y, Xue C, Wang S, Jin S, Zhu Z, et al.Prime genome editing in rice and wheat. Nat Biotechnol. 2024;38:582-585 pubmed publisher
WebbThis plasmid was modified from pKDL071 described in Litcofsky K.D., Afeyan R.B., Krom R.J., Khalil A.S., Collins J.J.; Iterative plug-and-play methodology for ... small limited companyWebb7 jan. 2024 · product information. Strains containing DP6 must be maintained in rich media supplemented with 25 mM glucose. Received from Ahmed Badran, former Liu lab member. high-stakes testsWebbpH-nCas9-PBE质粒价格1200元,pH-nCas9-PBE(爱迪基因 98163 )质粒图谱(map),质粒序列(Sequence)见下文,质粒抗性为卡那霉素。pH-nCas9-PBE质粒是植物表达;水稻。 high-strength htd timing belt idler pulleysWebbpH-nCas9-PPE-V2 Plasmid #170131 Purpose For plant prime editing in rice plants or monocotyledons protoplasts Depositor Caixia Gao Article Lin et al Nat Biotechnol. 2024 Mar 25. pii: 10.103 Insert nCas9 (H840A)-M-MLV Use CRISPR Tags Expression Plant Mutation H840A for Cas9; D200N, T306K, W313F, T330P and L… Promoter maize … high-strength basalt pipe insulationhttp://www.plantgenomeediting.net/ small lime tree winterWebbPlasmid pCre-AntiDsRed from Dr. James Birchler's lab contains the insert AntiDsRed Cre recombinase and is published in Unpublished This plasmid is available through Addgene. high-street-chainWebb16 mars 2024 · The pH-nCas9-PPE3 binary vectors were transformed into Agrobacterium tumefaciens strain EHA105 by electroporation. Callus cells of Zhonghua11 were … small limb chain saw